Optimize Your RNAseq
Optimize your research with our RNA library preparation service. We convert nucleic acid sequences into formats suited for high-precision sequencing, ensuring reliable and comprehensive results. Leverage our expertise for optimal transcriptomic analysis.
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Bulk RNA-seq allows for the quantification of total gene expression or all mRNA from a cell mixture, providing an overview of mRNA levels and transcriptional regulation within a sample. Our bulk RNA sequencing service provides you with the necessary data to advance your research.
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Single-cell RNA sequencing allows the analysis of gene expression at the individual cell level within a given population, simultaneously measuring the expression of hundreds to thousands of genes. This technique unveils the heterogeneity of cell populations, reconstructs cellular development trajectories, and models transcriptional dynamics, providing insights that were previously masked by bulk RNA sequencing methods.
GENXMAP offers a library preparation service from RNA, specially designed for total RNA sequencing (bulk) or single-cell RNA sequencing (sc-RNA seq) applications. With this advanced approach, it is possible to accurately quantify RNA levels, identify genes whose expression varies under different conditions, and explore complex processes like alternative splicing. By integrating these solutions into your projects, you gain access to robust and detailed gene expression analyses, whether for large-scale studies or research on individual cells.
GENXMAP ensures a deeper understanding of genetic mechanisms and significant progress in biomedical research.
Pure RNA, Unlocking Precision Analysis
GENXMAP, your trusted partner to support you in your research.
Service Details
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Identifying the Objectives
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Sample Type: Nature, quantity, condition, and availability of samples (purified RNA, cells, tissues, etc.). Envisioned Technologies: Advice on platform selection (Illumina).
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Sequencing Type Selection:
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Bulk RNA-seq (on total RNA, mRNA, microRNA)
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Single-cell RNA-seq
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Defining Parameters:
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Sequencing depth
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Genomic coverage
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Multiplexing (if applicable)
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Resource Validation:
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Evaluation of the quality and quantity of available samples.
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Advice on upstream preparation steps: extraction, purification, quality control.
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Defining the Budget:
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Discussion of fixed or flexible budget.
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Establishing the Timeline:
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Estimation of timeframes for each stage.
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Planning deliverables: raw data, analysis, reports.
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At the end of this consultation, you will receive:
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A detailed proposal: methods, technologies, budget, and timeline.
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A personalized strategy to maximize the success of your project.
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Clear guidance on how to prepare your samples and optimize results.
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High-throughput sequencing (Next-Generation Sequencing - NGS): GENXMAP uses the following platforms:
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NovaSeq (Illumina)
Advantages:
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Fast and accurate sequencing of large amounts of DNA from various environmental or biological sources.
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Provides high coverage and depth for precise detection of microorganisms present.
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MiSeq (Illumina)
Advantages:
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High accuracy: Illumina's synthesis sequencing technology is known for its low error rate, making it ideal for analyses that require high precision, such as genetic variation studies.
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Rapid turnaround: Quick run times enable timely results for urgent projects.
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Oxford Nanopore Technology
Advantages:
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Long reads: ONT allows the generation of very long reads (up to several hundred kb), which is valuable for studying complex genomic regions, whole genomes, and full transcripts without fragmentation.
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Real-time data: Immediate analysis as sequencing occurs, providing instant insights.
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Step 0 >> Sample Receipt Control
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Visual evaluation of quantity and quality.
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Report and client decision "Go / No-Go" for proceeding with the process.
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Step 1: Sample Preparation
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DNA/RNA Extraction: Optimized protocols ensure high-quality extraction, even from challenging samples (FFPE tissues, rare cells, etc.).
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Quantification and Quality Assessment: Verification of DNA/RNA quantity and quality using spectrophotometers (Nanodrop), Qubit, or Fragment Analyzer (Qiaxcel).
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Optional: mRNA enrichment via: i) ribosomal RNA removal, ii) polyA mRNA selection.
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For Single-Cell RNA Sequencing: Please refer to the specific page for the technique and process.
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Library Preparation: After DNA is extracted and optionally enriched, it is fragmented, and the necessary adapters for sequencing are added, creating a sequencing library ready for analysis on the chosen platform.
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Step 2 >> Sequencing Launch
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Based on the selected technology (Illumina), sequencing is performed on the appropriate platform.
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Step 3 >> Real-Time Quality Control
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Monitoring of the sequencing process to ensure the absence of technical errors and maintain the quality of the generated data.
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Step 4 >> Data Analysis
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We offer different levels of analysis: primary, secondary, or tertiary, depending on your needs. We collaborate with our partners to provide various types of analyses.
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For more details, please visit our "Bioinformatics & Biostatistics Analysis" page.
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Step 5 >> Validation and Interpretation
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Data Interpretation: Our bioinformatics experts and partners analyze the results to provide an in-depth interpretation. This analysis helps uncover complex relationships between microorganisms and identify potential biomarkers or therapeutic targets.
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Detailed Reports: A comprehensive report is generated, including result visualizations (GO pathways, UMAP, etc.), conclusions, and recommendations for the next steps based on project objectives.
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Step 6 >> Results Delivery
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Result Formats: Raw data files (FASTQ) provided.
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Detailed Report: Includes a summary of results, visualizations (based on requested analysis), biological interpretation, and recommendations for next steps.
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Delivery Methods: Access via a secure online platform, sent via email, or through a results presentation meeting.
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Follow-up and Support: Post-delivery assistance for clarifications, revisions, or additional analyses.
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Delivery Timeline: Typically between 3 to 6 weeks, depending on project complexity.
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Options
Access our sequencing solutions and obtain precise and in-depth transcriptomic analyses.
Select your approach:
Optimized and Transparent Services
Our Go/No-Go processes allow you to track the progress of your project in real-time. At each stage, you validate the transition to the next, ensuring complete control and maximum transparency throughout the entire process.
Our optimized timelines ensure fast and efficient execution of each project while meeting your specific requirements. This system ensures proactive time management, minimizing delays and maximizing productivity to deliver results that align with your expectations.
Our tailored processes are designed to meet the unique needs of each project, offering flexibility and personalized follow-up. This system ensures rigorous control while delivering accurate results that align with your expectations.
Our detailed analyses guarantee reliable and in-depth results tailored to each study. With cutting-edge tools and proven methodologies, we provide a precise understanding of genetic data, while adhering to the highest standards of quality and scientific rigor.
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