Transcriptomic Profiling: Unraveling Gene Expression for Biological Insights
Transcriptomic profiling enables the analysis of gene expression to better understand underlying biological processes, including metabolic pathway regulation, treatment responses, and the identification of specific biomarkers. These analyses are essential for exploring pathological mechanisms, optimizing therapeutic strategies, and characterizing molecular signatures across various contexts, from fundamental research to personalized medicine.
At GENXMAP, we offer multiple levels of transcriptomic profiling tailored to your needs:
A) Comprehensive Transcriptome Profiling – Exploratory Approach
A complete solution for biomarker discovery and global transcriptomic signature analysis using:
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Bulk RNA-sequencing for large-scale expression profiling
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Single-cell RNA-seq for cellular-level resolution
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Spatial Transcriptomics to preserve tissue architecture and spatial context
B) Targeted Transcriptome Profiling
A precise solution for transcript validation and quantification:
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RT-qPCR for relative quantification of gene expression
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Digital PCR (dPCR) for absolute quantification and detection of rare or low-expressed transcripts
Decoding Gene Expression, Unlocking New Insights
Global Approach: Integrated and Optimized Process
Precise and Actionable Analyses to Power Your Discoveries
A turnkey approach with personalized support,
from extraction to data analysis.
1. Sample Extraction and Quality Control
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Total RNA Extraction
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RNA Quality and Integrity Assessment (Bioanalyzer/Tapestation)
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Accurate Quantification (Qubit, Nanodrop)
2. Library Preparation
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Selection of RNA of Interest (PolyA, Ribo-depletion as needed)
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Library Construction Compatible with Illumina/NovaSeq Platforms
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Library Quality Control Before Sequencing
3. High-Throughput Sequencing
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Paired-End Sequencing 2x100 bp or 2x150 bp
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Depth Adjusted to Study Objectives (20 to 100 million reads per sample)
4. Bioinformatics and Biostatistical Analysis
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Quality Control of Reads (FastQC, Trimming)
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Alignment to the Reference Genome
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Quantification of Expression Levels (TPM, FPKM, CPM)
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Differential Expression Analysis (DESeq2, EdgeR)
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Functional Annotation and Pathway Enrichment
Customized Project and Multi-Omics Expertise
Options
Access our transcriptomic profiling solutions to better understand the underlying biological processes.
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GENXMAP, your trusted partner to support you in your research.
Optimized and Transparent Services
Our Go/No-Go processes allow you to track the progress of your project in real-time. At each stage, you validate the transition to the next, ensuring complete control and maximum transparency throughout the entire process.
Our optimized timelines ensure fast and efficient execution of each project while meeting your specific requirements. This system ensures proactive time management, minimizing delays and maximizing productivity to deliver results that align with your expectations.
Our tailored processes are designed to meet the unique needs of each project, offering flexibility and personalized follow-up. This system ensures rigorous control while delivering accurate results that align with your expectations.
Our detailed analyses guarantee reliable and in-depth results tailored to each study. With cutting-edge tools and proven methodologies, we provide a precise understanding of genetic data, while adhering to the highest standards of quality and scientific rigor.
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